Ibnosina Journal of Medicine and Biomedical Sciences

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 13  |  Issue : 4  |  Page : 196--203

Identification of Microorganisms associated with intraamniotic infection among women with preterm birth at Ruhengeri Referral Hospital, Rwanda: A case control study


Callixte Yadufashije1, Jasmine Umugwaneza1, Liliane Muhimpundu1, Cedrick Izere1, Emmanuel Munyeshyaka1, Albert O Mala2, Niyonzima N Francois1, Joseph Mucumbitsi1, Georges B Sangano3, Martin Ndayambaje4, Lydia Mwanzia5, Thierry Habyarimana1 
1 Department of Biomedical Laboratory Sciences, INES-Ruhengeri Institute of Applied Sciences, Ruhengeri, Rwanda
2 Department of Medical Laboratory Sciences, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya
3 Department of Nursing, School of Nursing and Midwifery, University of Rwanda, Huye, Rwanda
4 Department of Biology, Université Hassan II de Casablanca, Casablanca, Morocco
5 Department of Midwifery and Gender, Moi University, Eldoret, Kenya

Correspondence Address:
Dr. Callixte Yadufashije
Department of Biomedical Laboratory Sciences, INES-Ruhengeri-Institute of Applied Sciences, Ruhengeri
Rwanda

Background: Preterm birth is a global public health threat for maternal and child health. Each year, 15 million neonates are born preterm worldwide, with 40% resulting from intrauterine infections. Materials and Methods: This cross-sectional and case–control study was conducted from October to February 2019 at Ruhengeri Referral Hospital. A total of 120 swab samples were collected from 40 women, of which 20 were full-term delivery, while the other 20 were preterm delivery. The three samples, including the placental membranes, amniotic fluids, and fetal membranes, were collected immediately after birth. A sterile cotton swab was used to collect the samples and put into swab Stuart sterile plastic container to avoid sample contamination. Samples were transported in a tightly covered carrier to the clinical microbiology laboratory at INES Ruhengeri for microbiological investigation. Gram staining, culture, and biochemical tests were performed. The independent t-test was used to test for significant differences between the means of the two groups, while the Chi-square test (χ2) was used to test for significant association with microorganisms and intra-amniotic infections. Results: A half of the participants were in the age range of 24–29 years. Non-albicans candida (32.7%) and mold (27.9%) were the predominant microorganisms isolated. Non-albicans candida and mold were common to preterm and full-term samples. Staphylococcus species were observed in placental and fetal membrane samples. Escherichia coli, Klebsiella species, Streptococcus species, and Candida albicans were observed among women with preterm birth samples. There was a statistically significant difference between the two means in the amniotic fluid isolates (t = 4.023, P < 0.007), placental membrane isolates (t = 7.17, P < 0.0004), and fetal membrane isolates (t = 6.7, P < 0.0006). Association with microorganisms and intra-amniotic infection was statistically significant with E. coli2 = 3.98, P < 0.05), Streptococcus species (χ2 = 5.53, P < 0.019), non-albicans candida (χ2 = 8.37, P < 0.004), and C. albicans2 = 3.98, P < 0.05). Conclusions: Invasion of the amniotic fluid, placenta, and fetal membranes by pathogenic microorganisms may be associated with the incidence of preterm labor and birth. Early diagnosis is recommended to avoid both maternal and fetal complications.


How to cite this article:
Yadufashije C, Umugwaneza J, Muhimpundu L, Izere C, Munyeshyaka E, Mala AO, Francois NN, Mucumbitsi J, Sangano GB, Ndayambaje M, Mwanzia L, Habyarimana T. Identification of Microorganisms associated with intraamniotic infection among women with preterm birth at Ruhengeri Referral Hospital, Rwanda: A case control study.Ibnosina J Med Biomed Sci 2021;13:196-203


How to cite this URL:
Yadufashije C, Umugwaneza J, Muhimpundu L, Izere C, Munyeshyaka E, Mala AO, Francois NN, Mucumbitsi J, Sangano GB, Ndayambaje M, Mwanzia L, Habyarimana T. Identification of Microorganisms associated with intraamniotic infection among women with preterm birth at Ruhengeri Referral Hospital, Rwanda: A case control study. Ibnosina J Med Biomed Sci [serial online] 2021 [cited 2022 May 26 ];13:196-203
Available from: http://www.ijmbs.org/article.asp?issn=1947-489X;year=2021;volume=13;issue=4;spage=196;epage=203;aulast=Yadufashije;type=0